the effect of the antioxidant drug U-74389G on endometrial edema during ischemia reperfusion injury in rats

The aim of this experiment was to study the effects of the antioxidant drug U-74389G on rat model, particularly in ischemia reperfusion (IR) protocol. The beneficial or other effects of that molecule were studied pathologically using endometrial edema (EE) lesions. Forty rats were used of mean weight 231.875 gr. EE lesions were evaluated 60 min after reperfusion (groups A and C) and 120 min after reperfusion (groups B and D), with administration of the drug U-74389G in groups C and D. Results were that U-74389G administration non-significantly decreased without lesions the EE scores by 0.41 [-1.00 0.17] (p= 0.1607). This finding was in accordance with the results of Wilcoxon signed-rank test (p= 0.5229). Reperfusion time nonsignificantly increased without lesions the EE scores by 0.21 [-0.38 0.81] (p= 0.4701), also in accordance with Wilcoxon signed-rank test (p= 0.1022). However, U-74389G administration and reperfusion time together non-significantly decreased without lesions the EE scores by 0.17 [-0.53 0.18] (p= 0.3383). Results of this study indicate that U-74389G administration hardly non-significantly decreases without lesions the EE within short-term time context of 2 hours. Acta Biol Szeged 58(1):69-72 (2014) Key WordS antioxidant drug endometrial edema reperfusion U-74389G Accepted July 31, 2014 *Corresponding author. E-mail: [email protected] 69 Tissue ischemia and reperfusion (IR) remain one of the main causes of permanent or transient damage with serious implications on adjacent organs and certainly on patients’ health. The use of antioxidant substances has been a research subject for many years. However, even if important progress has been made, satisfactory answers have not been given yet to fundamental questions, such as, how much powerful should an antioxidant be, when should it be administered, and in which dosage. The particularly satisfactory action of the antioxidant U-74389G in tissue protection has been noted in several performed experiments. Since a careful literature search (PubMed Medline) was conducted, it was realized that this certain antioxidant has been tried in IR experiments. However, just few relative reports were found, not covering completely this particular matter. Also, a lot of publications addressed trials of other similar molecules of aminosteroids (lazaroids) to which the studied molecule also belongs to. U-74389G or better 21-[4-(2,6-di-1-pyrrolidinyl4-pyrimidinyl)-1-piperazinyl]-pregna-1,4,9(11)-triene-3,20dione maleate salt (Cayman Chemical Product Catalog) is an antioxidant which prevents both arachidonic acid-induced and iron-dependent lipid peroxidation. It protects against IR injury in animal heart, liver, and kidney models. These membrane-associating antioxidants (Shi et al. 1995) are particularly effective in preventing permeability changes in brain microvascular endothelial cells monolayers. The same authors (Tsompos et al. 2014) found a light non-significant decline in chloride serum levels in related IR injury experiments in rats by 0.58%+0.77% (p=0.4533) 1h after reperfusion, by 0.97%+0.53% (p=0.0879) 1.5h after reperfusion, by 0.75%+0.38% (p=0.0159) after time and drug interaction and by 1.36%+0.76% (p=0.1113) 2h after reperfusion. The aim of this experimental study was to examine the effect of the antioxidant drug U-74389G on rat model and particularly in a uterus IR protocol. The beneficial effect or non-effectiveness of that molecule was studied by evaluating mean endometrial edema (EE) lesions. Materials and Methods